The Ghana Football Association has invited 27 home-based players for camping ahead of next month's tournament in Brazil.
The players are to report at the Len Clay Stadium in Obuasi on 31 December for training.
Berekum Chelsea and Asante Kotoko dominate the list with four players each.
Head coach Dauda Luttrodt will slash the number to 20 for the assignment in Sao Paulo.
Local Black Stars squad:
Goalkeepers: Stephen Adams (Aduana) Foli Adade (Dwarfs) and Robert Dabou (Wa All Stars)
Defenders: Godfred Saka (Aduana) Christopher Bonney and Francis Morton (Dwarfs) Joshua Otoo (Wa All Stars) Ahmed Adams (Berekum Chelsea) Kwabena Adusei (Kotoko) Otuo Acheampong (Medeama) and Nuru Sulley (Hearts of Oak)
Midfielders: Jackson Owusuand Jordan Opoku (Berekum Chelsea) Seidu Salifu and Emmanuel Gyamfi (Wa All Stars) Issah Yakubu, Richard Mpong and Michael Helegbe (Kotoko) Prince Boateng (King Faisal) Theophilus Anobaah (Medeama) Aaron Amoah (AshantiGold) Moses Odjer (Tema Youth) and Kennedy Ashia (Liberty Professionals)
Attackers: Yakubu Mohammed (AshantiGold) Mahatma Otoo (Hearts of Oak) Latif Salifu (Berekum Chelsea) and Bismark Asiedu (Medeama)