Ghana duo Michael Essien and Sulley Muntari was excluded from the AC Milan set-up which lost 3-0 to Olympiacos at the BMO Field of Toronto.
The Black Stars players have been part of the Rossoneri pre-season squad in the USA.
It only took 16 minutes for the Greek side to take the lead when a cross found Dominguez all alone in the box and he beat Gabriel with a right foot finish.
Poli came close to equalising on 30 minutes but was just inches away from turning the ball into the back of the net but it was Olympiacos who continued to be the more dangerous side throughout the first half.
And just four minutes into the second period Olympiacos doubled their lead when Diamantakos beat Bonera to the cross.
The Rossoneri responded immediately with Saponara but his turn and shot only found the base of the post and the ball did not cross the line.
Milan kept pushing forward in search of a goal and had their chances from a series of corners but Inzaghi's men failed to find the back of the net and were punished again on 78 minutes when Bouchalakis unleashed an unstoppable shot from 30 metres out that finished in the top corner.