AshantiGold and Inter Allies are facing match-fixing charges and possible demotion to a lesser league if found guilty.
The two clubs are being investigated by Ghana Football Association and police after a player involved in their Ghana Premier League encounter made serious allegations of match-fixing.
The match ended 7-0 with Inter Allies defender, who is the centre of police investigation, scoring two deliberate own goals.
The two clubs if found guilty could be demoted to the fourth division plus a hefty fine, with Kurt Okraku's GFA administration determined to protect the integrity of the country's number one sport.
AshantiGold finished the season 9th hence are still a Ghana Premier League club, meanwhile, Inter Allies have been relegated.
In 2007, Nania FC, Okwahu United, Great Mariners FC and Tudu Mighty Jets were demoted to the fourth division after they were found guilty of match-fixing.
Nania and Mariners were level on points in their group and needed to win their final matches to qualify for the country's top league.
Abedi Pele's Nania FC beat Okwawu United 31-0 while Great Mariners triumphed 28-0 over Mighty Jets in league games played earlier this year.
However, upon successful appeals, the four clubs maintained their second-tier status but severed one season bans.