Diego Costa now has 15 days to appeal RFEF's decision on Friday.
Atletico Madrid have failed in their bid to overturn Diego Costa's eight-game ban for insulting referee Jesus Gil Manzano's mother in last Saturday's defeat to Barcelona.
The Royal Spanish Football Federation (RFEF) appeals committee turned down Atletico's appeal on Friday and confirmed the suspension handed to Costa by the competition committee.
Watch Serie A, MLS, Nations League, FA Cup and more streamed live on ESPN+ in U.S.
Atletico have 15 days to appeal to the Administrative Court for Sport (TAD).
The Spain international was shown a straight red card in the 28th minute of the 2-0 loss after he approached Gil Manzano in the middle of the pitch and shouted in his face.
The details of his outburst were included the referee's report following the game and the audio of the incident was reviewed by the league's refereeing committee.
The Spanish FA sanctioned Costa four matches for his comments to the referee and added another four games of sanction after Costa was seen grabbing Gil Manzano's arm after the red card was shown.
Costa will miss the rest of the season as Atletico only have seven La Liga fixtures remaining.
Last Saturday's defeat to title rivals Barcelona left Diego Simeone's side 11 points adrift of the league leaders, while Atletico have also been eliminated from the Champions League and the Copa del Rey.
Source: espn.co.uk