Hearts of Oak coach Nebojsa Vucicevic was prevented from training with the squad on Friday morning by angry fans.
Some irate supporters besieged the team's ATTC training Park in Accra and physically stopped him from performing his duties.
Their action is in protest of the club's recent 1-0 defeat at AshantiGold which has thrown their title challenge in doubt.
The club's supportersâ€™ body, National Chapters Committee, sent an official correspondence to the management on Wednesday demanding Vucicevic's immediate dismissal.
They have expressed dissatisfaction with the Serbianâ€™s tactics and player selection.
The latest development could be the last straw for Vucicevic who could be sacrificed to appease the angry supporters.
Hearts trail leaders and rivals Asante Kotoko by seven points with 12 matches remaining in the Ghana top flight.
Management is yet to decide on the demands from the supporters as the coach's dismissal might come with a huge severance package.