The top-rated midfielder made his last appearance for the side as they threw away a two-goal lead to lose 3-2 at Schale 04 to miss out on Bundesliga promotion.
Fans of the club will not see the somersault celebration of the highly-rated Ghanaian at the Millerntor-Stadion next season.
The 26-year-old will not be short of offers with a number of top Bundesliga clubs hot on his heels for his signature.
Daniel-Kofi Kyereh will miss the final game of the season after picking a fifth booking in the match against Schalke, ruling him out of the Fortuna Dusseldorf clash.
The Ghana international was cautioned in the matches against Dresden, Heidenheim, Hansa Rostock, Werder Bremen and Darmstadt.
This means Kyereh will not be getting a farewell as he leaves the club at the end of the season following two successful campaign.
The former Wehen Wiesbaden player made 67 appearances for St Pauli, scoring 22 goals and providing 21 assists.