Real Tamale United crashed out of the MTN Ghana FA Cup against lower division side Rainbow FC on Saturday.
The Pride of the North drew 1-1 with home side but got bundled out 3-1 penalties.
Ahead of Sunday’s round of matches, RTU have become the major casualty at the round of 64 stage.
Premier League side Inter Allies edged Kristal Palace 1-0 and Hasaacas secured a 2-0 win at Holy Stars.
High-flying Okyeman Planners triumphed 4-1 on penalties after drawing 2-2 at Manya Red Lions.
SATURDAY
STK Stars 1-2 Asokwa Deportivo
Kristal Palace 0-1 Inter Allies
Holy Stars 0-2 Hasaacas
Dunkwa United 2-0 Lions
Rainbow 1-1(pen 3-1) RTU
Rocket 1-0 Bawku United
Purejoy 2-0 Danbort
Unicon 0-1 True Democracy
Manya Red Lions 2-2 (pen 1-4) Okyeman Planners
FRIDAY
Sunyani Liberty 2-0 Tamale Liberty
Karela FC 1-0 SP Mirren
Efutu Gt Kingdy 0-2 WAFA
Power Sporting 0-1 Nsuatre