Atalanta replaced Papu Gomez and Johan Mojica with Bosko Sutalo and Joakim Maehle in the squad registered for the knockout stages in the Champions League.
The 21-year-old Croatian and the 23-year-old Dane have been registered for the last 16 in the European competition.
Attacking midfielder Viktor Kovalenko, who played three times in the tournament with Shakhtar Donetsk, is not available for the match against Real Madrid on February 24.
Atalanta squad for the Champions League: Gollini, Sportiello, Rossi; Caldara, Djimsiti, Gosens, Hateboer, Maehle, Palomino, Romero, Sutalo, Toloi; De Roon, Freuler, Malinovskyi, Miranchuk, Pasalic, Pessina; Ilicic, Lammers, Muriel, Zapata
Source: m.allfootballapp.com