A section of Lazio's stadium will be closed for two Serie A games following racist chanting directed at Ghana midfielder Alfred Duncan by fans during the 6-1 home win against Sassuolo on Sunday.
Chants were aimed at Sassuolo's Claud Adjapong and Alfred Duncan.
Serie A's disciplinary report said "2,000 of the 5,449 occupants" of the Curva Nord stand were guilty of "chants expressive of racial discrimination".
The Curva Nord will be closed for the visit of Cagliari on 22 October and Udinese on 5 November.
The game at Stadio Olimpico on 5 November was added as Lazio were already serving a suspended sentence for the same offence after the Rome derby on 30 April.