The left-back has signed a three-year deal with the two-time FA Cup champions.
He becomes the latest signing by the club following the arrival of Zakaria Mumuni, Benjamin Arthur, Coulibaly Zana Oumar, Amed Toure and Vincent Atinga.
Jabila has had a previous stint with the Mauve and Yellows.
"I am very happy to return to Medeama. I have an unfinished business and hope to complete it in style," Jabila told the club's website.
"I can promise that I will give everything in my heart to be a success here.
"Medeama is a great club with huge ambition and I am delighted to be part of this fantastic group."
We are delighted to announce the signing of versatile Abdul Karim Jabila on a three-year deal.
He previously played for Legon Cities.
— Medeama SC (@MedeamaSC) March 18, 2021
Jabila featured for Legon Cities during the first half of the season.
But he has been snapped by coach Yaw Preko for the next three seasons in Tarkwa.